Thioacetamide-induced liver fibrosis is reduced in humanized immune system mice

Abstract Fibrosis is defined as a consequence of the abnormal wound-healing response lading to excessive deposition extracellular matrix components. Chronic liver diseases accompanied with fibrosis have caused increased morbidity and mortality worldwide. Carbon tetrachloride (CCl 4) thioacetamide (TAA) are well-known toxins used induce in murine. Compared CCl 4, TAA displays longer latency between exposures drug less safety concerns operators. In addition, translation murine experimental data human events often fails due differences immune system both species, indicating that humanized mice may be better choice model diseases. The effect still unknown. our study, wild type was induced by escalating doses TAA. Results showed evaluated Sirius red staining significantly (Metavir score, F2). serum levels AST, ALT ALP were after injection. Humanized generated injection hematopoietic stem cells (hHSC) into NOD.Cg-PrkdcscidIl2rgtm1Wjl/YckNarl (Advanced Severe ImmunoDeficiency (ASID)) mice, termed hHSC-ASID. introduced hHSC-ASID ASID mice. Although following injection, degree reduced than F1 vs. These results indicated TAA-induced fibrosis. It worthwhile assess which cell(s) participates this process.